Welcome to Disha Life Sciences Pvt. Ltd
Module Course/Technique Course Detail
1 Molecular Biology Plant DNA isolation, plasmid isolation and qualitative and quantitative estimation by spectrophotometer and qubit flurometer, agarose gel electrophoresis
2 Southern blotting Agarose gel electrophoresis, reagent preparation and blotting
3 Transformation Plasmid isolation, competent cells preparation, transformation by alkaline lysis method and electrophoresis
4 Polymerase Chain Reaction PCR handling and programming, Amplification, Agarose gel electrophoresis of PCR products
5 Protein Isolation Isolation of crude protein from plant, protein precipitation , qualitative analysis by spectrophotometer
6 Proteomics Protein estimation by Lowry’s and Bradford method, SDS and Native gel electrophoresis
7 ELISA Principle , introduction of ELISA reader, dilution preparation, coating , blocking and reading of microtitre plate
8 High performance liquid chromatography (HPLC) Principles and Introduction of HPLC, Sample preparation, injection and Troubleshooting
9 Gas chromatography(GC) Principle of GC, System operation, Sample preparation, injection and Troubleshooting
10 Ion exchange chromatography Principle , reagent preparation, column packing, purification of enzyme, estimation of enzyme activity and calculation