| 1 |
Molecular Biology |
Plant DNA isolation, plasmid isolation and qualitative and quantitative estimation by spectrophotometer and qubit flurometer, agarose gel electrophoresis |
| 2 |
Southern blotting |
Agarose gel electrophoresis, reagent preparation and blotting |
| 3 |
Transformation |
Plasmid isolation, competent cells preparation, transformation by alkaline lysis method and electrophoresis |
| 4 |
Polymerase Chain Reaction |
PCR handling and programming, Amplification, Agarose gel electrophoresis of PCR products |
| 5 |
Protein Isolation |
Isolation of crude protein from plant, protein precipitation , qualitative analysis by spectrophotometer |
| 6 |
Proteomics |
Protein estimation by Lowry’s and Bradford method, SDS and Native gel electrophoresis |
| 7 |
ELISA |
Principle , introduction of ELISA reader, dilution preparation, coating , blocking and reading of microtitre plate |
| 8 |
High performance liquid chromatography (HPLC) |
Principles and Introduction of HPLC, Sample preparation, injection and Troubleshooting |
| 9 |
Gas chromatography(GC) |
Principle of GC, System operation, Sample preparation, injection and Troubleshooting |
| 10 |
Ion exchange chromatography |
Principle , reagent preparation, column packing, purification of enzyme, estimation of enzyme activity and calculation |
