• Antigen- antibody testing by ELISA
• Double Immunodiffusion
• RH Typing of Blood sample
• Radial Immunodiffusion
• WIDAL Test
• Rocket Immuno Electrophoresis
• Counter current electrophoresis
• Gel diffusion techniques
• Agglutination Reactions
• Rapid Plasma Reagin Test (RPR)
• Raising of polyclonal antibodies
• Monoclonal antibody production
• Enzyme Assay
Screening of purified cultures for Enzyme activity.
Extraction & Fermentation of crude enzyme
Precipitation of enzyme
Characterization of Purified Enzyme
Total protein estimation
I.Bradfords Method.
II.Lowrys Method.
Enzyme Assay
Screening of purified cultures for Enzyme activity.
• Protein concentration
Kjeldahl method
UV- Visible spectroscopy method.
• Protein separation
Dialysis
Ion exchange chromatography
Electrophoresis